Evaluation of mixed-source, low-template DNA profiles in forensic science
نویسندگان
چکیده
منابع مشابه
Evaluation of mixed-source, low-template DNA profiles in forensic science.
Enhancements in sensitivity now allow DNA profiles to be obtained from only tens of picograms of DNA, corresponding to a few cells, even for samples subject to degradation from environmental exposure. However, low-template DNA (LTDNA) profiles are subject to stochastic effects, such as "dropout" and "dropin" of alleles, and highly variable stutter peak heights. Although the sensitivity of the n...
متن کاملInterpreting low template DNA profiles.
We discuss the interpretation of DNA profiles obtained from low template DNA samples. The most important challenge to interpretation in this setting arises when either or both of "drop-out" and "drop-in" create discordances between the crime scene DNA profile and the DNA profile expected under the prosecution allegation. Stutter and unbalanced peak heights are also problematic, in addition to t...
متن کاملA Review of the Science of Low Template DNA Analysis
From our detailed review we find that the science supporting the delivery of Low Template DNA (LTDNA) analysis is sound and that the three companies (the Forensic Science Service Ltd, LGC Forensics and Orchid Cellmark Ltd) providing this service to the Criminal Justice System have validated their processes in accord with accepted scientific principles using both 28 and 34 PCR cycles for extract...
متن کاملDNA and Forensic Science
This paper represents a discussion of the fundamental principles of DNA technology as it applies to forensic testing. A brief overview of DNA science will be given to clarify the terminology and techniques described for forensic and basic analysis. A historical as well as scientific discussion of the molecular biological tools used to determine DNA profiles and database analysis following such ...
متن کاملDNA Profiling in Forensic Science
The discovery of hypervariable minisatellite deoxyribonucleic acid (DNA) by Jeffreys and co-workers in 1985 provided forensic scientists with a means of discriminating between individuals that was many orders of magnitude greater than the traditional methods used before that date. Probably themost important forensic application of DNA profiling is in the identification of rapists. The original ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2013
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1219739110